High-Grade Glandular Dysplasia Can Be Confirmed By Positive Staining With Progenitor Cell Marker CD133

Document Type

Conference Proceeding

Publication Date

9-2018

Publication Title

Archives of Pathology and Laboratory Medicine

Abstract

Context: Our group and others have previously shown that colonic adenocarcinoma overexpresses CD133. High-grade glandular dysplasia (HGGD) is a well-recognized precursor of invasive colonic adenocarcinoma, defined by architectural changes (cribriform pattern) and cytologic atypia (rounding of nuclei in dysplastic cells). The aim of our current study was to investigate whether CD133 can be used to distinguish HGGD with or without significant cytologic atypia from low-grade dysplasia.

Design: We collected 20 nonneoplastic cases with reactive bowel tissue (10 ischemic and 10 inflammatory bowel disease), 12 cases with HGGD (8 with and 4 without significant cytologic atypia), 8 cases with only low-grade glandular dysplasia, and 10 cases of invasive adenocarcinoma. All cases were stained with CD133 (AC 133 monoclonal antibody); surface membranous staining was evaluated and graded from 0 to 3+.

Results: CD133 staining was negative in all reactive bowel cases, whereas all colonic adenocarcinomas showed 2–3+ positivity. All cases of HGGD displayed 2–3+ surface membranous staining, both with and without cytologic atypia. CD133 staining was almost entirely negative in cases with only low-grade dysplasia, aside from 1 case with focal positive surface membranous staining.

Conclusions: Although HGGD with cytologic atypia can be easily identified morphologically, HGGD without significant cytologic atypia can be further supported by evidence of positive surface membranous staining for CD133. Meanwhile, low-grade dysplasia, either as individual cases or between areas of HGGD, stains almost exclusively negative for CD133. In summary, our study indicates that CD133 surface expression closely correlates with HGGD.

Volume

142

Issue

9

First Page

e35

Comments

College of American Pathologists Annual Meeting, October 20-24, 2018, Chicago, IL

Last Page

e36

DOI

10.5858/arpa.2018-0293-AB

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