The Finer Aspects of Grid-Walking and Cylinder Tests for Experimental Stroke Recovery Studies in Mice

Authors

• Nausheen Syeara
• Sounak Bagchi
• Abdullah Al Shoyaib
• Serob T Karamyan, Oakland University William Beaumont School of Medicine Faculty
• Faisal F Alamri
• Vardan T Karamyan, Oakland University William Beaumont School of Medicine Faculty

Document Type

Article

Publication Date

1-1-2023

Publication Title

Methods in molecular biology (Clifton, N.J.)

Abstract

The choice of behavioral tests and their proper execution is critically important for experimental and preclinical therapeutic stroke recovery studies, where improvement of impaired neurological function(s) is the main outcome measure. Two tests that focus on spontaneous motor behaviors of the forelimb during gait and exploratory rearing and are expert recommended for stroke recovery studies in mice are grid-walking and cylinder tasks. Both tests have been widely used in various experimental stroke studies to evaluate acute and chronic motor impairment. To facilitate adoption of these tests and consistency of use between different research laboratories, this chapter describes a simple and rigorous protocol and our schemes to successfully perform both tasks in mice and evaluate motor dysfunction and recovery after stroke. In addition, we provide practical tips to minimize experimental bias and acquire data for analyses.

Volume

2616

First Page

345

Last Page

353

Recommended Citation

Syeara N, Bagchi S, Al Shoyaib A, Karamyan ST, Alamri FF, Karamyan VT. The Finer Aspects of Grid-Walking and Cylinder Tests for Experimental Stroke Recovery Studies in Mice. Methods Mol Biol. 2023;2616:345-353. doi:10.1007/978-1-0716-2926-0_23

DOI

10.1007/978-1-0716-2926-0_23

ISSN

1940-6029

PubMed ID

36715944