A Low-Budget Photothrombotic Rodent Stroke Model

Authors

• Faisal F Alamri
• Serob T Karamyan, Oakland University William Beaumont School of Medicine Faculty
• Vardan T Karamyan, Oakland University William Beaumont School of Medicine Faculty

Document Type

Article

Publication Date

1-1-2023

Publication Title

Methods in molecular biology (Clifton, N.J.)

Abstract

A number of animal stroke models have been developed and used over the years to study the pathological mechanisms of this disorder and develop new therapies. Among them, the photothrombotic model of ischemic stroke has been central in various studies focusing on understanding of the basic biology of neural repair, identification and validation of key molecular targets involved in post-stroke recovery, and preclinical testing of various therapeutic approaches. To facilitate uniformity among various experimental groups using this expert-recommended mouse model of choice for stroke recovery studies, in this chapter we describe in detail a low-budget technique to induce photothrombosis in the mouse primary motor cortex. Additionally, we provide tips for conducting this procedure in other cerebral cortical regions of the mouse brain and in rats.

Volume

2616

First Page

21

Last Page

28

Recommended Citation

Alamri FF, Karamyan ST, Karamyan VT. A Low-Budget Photothrombotic Rodent Stroke Model. Methods Mol Biol. 2023;2616:21-28. doi:10.1007/978-1-0716-2926-0_3

DOI

10.1007/978-1-0716-2926-0_3

ISSN

1940-6029

PubMed ID

36715924