Acute local and systemic effects of intra-articular administration of liposomal Ido-1 following anterior cruciate ligament rupture.

Document Type

Article

Publication Date

7-28-2026

Publication Title

Biochimie

Abstract

Joint injuries, such as rupture of the anterior cruciate ligament (ACL), are associated with the development of post-traumatic osteoarthritis (PTOA). ACL rupture can lead to disruption of metabolic pathways, including the conversion of tryptophan to kynurenine, which is associated with a sustained inflammatory response. An in vivo study was undertaken to determine the acute effects of intra-articular administration of liposomes loaded with the tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase-1 (IDO-1) following ACL rupture. Using a rat model of non-surgical ACL injury, male and female rats underwent a single intra-articular injection of empty liposomes, or liposomes loaded with IDO-1 and were subsequently randomized to 1- or 2-week endpoints. IDO-1 treatment after ACL injury was associated with a significant reduction in synovial fluid concentration of tryptophan at both 1- and 2-week endpoints. In addition to reduced tryptophan, IDO-1 treatment led to significantly lower synovial fluid concentrations of IL-1β and TNF-α. IDO-1-loaded liposomes also increased the ratio of regulatory T lymphocytes (Tregs) to IL-17-secreting helper T lymphocytes (Th17 cells). Similarly, IDO-1 treatment increased the number of CTLA4

Volume

S0300-9084

Issue

26

First Page

00173-2

DOI

10.1016/j.biochi.2026.07.013

ISSN

1638-6183

PubMed ID

42520881

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